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Plat-A cells (8×105 cells/well) had been seeded 24h before transfection in 6-well plates in 2 mL/well of an infection medium (Plat-A medium without antibiotics). 20-24h later, once the cells had reached 70% confluence, 500μL/well of a mix containing plasmids and Lipofectamine were added. The mixtures contained 3 μg/well of transgene carrying plasmid, 2 μg/well of pMD2.G and 10 μL/well of Lipofectamine 2000, and had been prepared in OPTIMEM medium based on the manufacturer´s protocol.

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Dead cells have been excluded from the evaluation using Maleimide . Samples have been acquired with a Cytoflex cytometer and data analyzed utilizing FlowJo software. MC703 cells have been transduced twice with retrovirus-containing supernatants in the presence of polybrene (10 μg/mL) .




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